M4280

Anti-Mouse IgG (Fc specific) antibody produced in goat

2.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti Mouse Fc Antibody, Anti Mouse Fc Antibody - Anti-Mouse IgG (Fc specific) antibody produced in goat
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162625
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i)
• Citations: 22

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• concentration: 2.0 mg/mL
• technique(s): indirect ELISA: 1:20,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Immunogen

Fc fragment of IgG purified from mouse.

Application

Anti-Mouse IgG (Fc specific) antibody produced in goat has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• flow cytometry
• immunoprecipitation
• immunoblotting

Biochem/physiol Actions

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. The antigen binding activity is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain. Anti-mouse IgG (Fc specific) antibody can be used to detect the optimal capture agents.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable