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L0259

Lucifer Yellow CH dilithium salt

Powder

Synonym(s):

6-Amino-2,3-dihydro-1,3-dioxo-2-hydrazinocarbonylamino-1H-benz[d,e]isoquinoline-5,8-disulfonic acid dilithium salt

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About This Item

Empirical Formula (Hill Notation):
C13H9Li2N5O9S2
CAS Number:
Molecular Weight:
457.25
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
267-047-9
MDL number:
Beilstein/REAXYS Number:
4644601
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Product Name

Lucifer Yellow CH dilithium salt, fluorescent stain

Quality Segment

form

powder

color

faint orange to very dark orange

solubility

H2O: 1 mg/mL

fluorescence

λex 428 nm; λem 540 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Li+].[Li+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

RPKCZJYDUKVMGF-UHFFFAOYSA-L

General description

Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. Lucifer Yellow CH dilithium salt is a fluorescent dye(1) comprising of amino, sulfo and phenyl-N-hydrazinocarbonylamino substituents on the naphthalimide chromophore. The hydrophilic nature of Lucifer yellow CH underlies its numerous applications as a stain of living cells and tissues.

Application

A highly fluorescent dye, useful in marking nerve cells.
Lucifer yellow CH has been used to stain nerve cells[1] and to demonstrate electrical coupling between hamster oocytes and cumulous cells during meiotic maturation in vivo and in vitro.
Lucifer Yellow CH is mainly used as a tracer dye and useful in marking nerve cells.

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1 of 1

This Item
L3510L0144L6882
fluorescence

λex 428 nm; λem 540 nm in H2O

fluorescence

λex 428 nm; λem 536 nm in H2O

fluorescence

λex 428 nm; λem 540 nm

fluorescence

λex 328 nm; λem 532 nm in H2O

color

faint orange to very dark orange

color

yellow to brown

color

orange

color

-

solubility

H2O: 1 mg/mL

solubility

-

solubility

-

solubility

H2O: soluble

form

powder

form

powder

form

powder

form

lyophilized powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Related Content


Mariane C Vicente et al.
Experimental neurology, 328, 113250-113250 (2020-02-24)
The locus coeruleus (LC) is a pontine nucleus important for respiratory control and central chemoreception. It is affected in Alzheimer's disease (AD) and alteration of LC cell function may account for respiratory problems observed in AD patients. In the current
Metabolic, fluorescent dye and electrical coupling between hamster oocytes and cumulus cells during meiotic maturation in vivo and in vitro.
Racowsky, C. and Satterlie, R.A.
Developmental Biology, 108, 191-202 (1985)
Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer.
W W Stewart
Cell, 14(3), 741-759 (1978-07-01)



Global Trade Item Number

SKUGTIN
L0259-100MG04061833945612
L0259-250MG04061833945629
L0259-25MG04061833945636

Questions

  1. What is the dye purity for product L0259?

    What is the dye purity for product L0259?

    1 answer
    1. At M-Clarity quality level 100, Product L0259 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0259#product-documentation

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