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I4036

IgA from human serum

reagent grade, ≥95% (HPLC), buffered aqueous solution

Synonym(s):

Human IgA

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Assay:
≥95% (HPLC)
Form:
buffered aqueous solution
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conjugate

unconjugated

Quality Segment

300

grade

reagent grade

assay

≥95% (HPLC)

form

buffered aqueous solution

storage temp.

−20°C

General description

IgA is one of the most abundant antibody isotypes produced in the body. It is mainly secreted by plasma cells located in the lamina propria. There are two subtypes of IgA, IgA1 and IgA2 present primarily in the airways and colon, respectively.

Application

IgA from human serum has been used in enzyme-linked immunosorbent assay (ELISA) for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-spike-receptor binding domain (RBD) antibody detection in blood samples.
Purified human IgA may be used to study its physiological and biological effects on processes such as inflammation, pathogen elimination, regulation of antibody-dependent-cell-mediated cytotoxicity (ADCC), modulation of phagocytosis, degranulation of eosinophils and basophils and modulation of respiratory burst activity.

Biochem/physiol Actions

IgA aids in inducing a defense mechanism against various viral pathogens such as rotavirus, poliovirus, influenza virus, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It regulates excessive immune responses in inflammatory diseases. IgA also has the potential to trigger neutrophil recruitment for cell killing.

Physical form

Solution in 0.01 M Phosphate Buffer saline, pH 7.4, containing 15 mM sodium azide as a preservative.

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This Item
I2511401098I8260
IgA from human serum reagent grade, ≥95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I4036

IgA from human serum

IgG from human serum reagent grade, ≥95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I2511

IgG from human serum

Human IgA, Plasma

Sigma-Aldrich

401098

Human IgA, Plasma

IgM from human serum reagent grade, ~95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I8260

IgM from human serum

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (SDS-PAGE)

assay

~95% (HPLC)

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

form

buffered aqueous solution

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

300

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

grade

reagent grade

grade

reagent grade

grade

-

grade

reagent grade

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–2 of 2 Questions  

  1. What's the volume of the solution in each vial? What's the stability of the stock solution and after aliquoting and storing at -20ºC or -80ºC? Thank you.

    1 answer

    1. The concentration of this product may range from 0.8 – 1.2 mg/mL. This information is reported in the lot-specific Certificate of Analysis. The minimum and maximum volume of each package size it listed below:
      1 mg volume range – 0.83 mL minimum – 1.25 mL maximum
      5 mg volume range – 4,16 mL minimum – 6.25 mL maximum

      This material is quite stable when stored at -20ºC . The product is assigned a retest date of every two years following the date of quality release. Stored aliquots are expected to be stable at least until the retest date published in the lot-specific Certificate of Analysis.
      Please see the following link to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i4036#product-documentation

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/147/322/i4036dat.pdf

      Helpful?

  2. What is the isoform of the IgA? Is it only in its dimeric form or is it a mix of the monomer and the dimer?

    1 answer

    1. Human IgA exists in 2 forms. It is a monomer in the serum and a dimer in mucosal secretory tissues [secretory IgA (sIgA)]. Secretory IgA is a complex comprising two IgA monomers, a connecting J chain, and a secretory component.

      Helpful?

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