G3153

β-Galactosidase from Escherichia coli

lyophilized powder, ≥500 units/mg protein

• Synonym(s): β-D-Galactoside galactohydrolase, Lactase
• CAS Number: 9031-11-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-864-1
• MDL number: MFCD00155434
• EC Number: 3.2.1.23 (BRENDA, IUBMB)
• Specific activity: ≥500 units/mg protein
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 465 kDa
• storage temp.: −20°C

Description

General description

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Application

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.[1]

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.[2]

Packaging

Package size based on protein content

Physical form

Stabilized with phosphate buffer and sucrose

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)