Lambda DNA EcoR I Hind III Digest

Name: Lambda DNA <I>Eco</I>R I <I>Hind </I>III Digest
Brand: SIGMA

for DNA electrophoresis

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About This Item

NACRES:

NA.25

UNSPSC Code:

41141827

Form:

liquid

Storage temp.:

−20°C

Pricing and availability is not currently available.

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Properties

Quality Level

100, 200

form

liquid

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Description

General description

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Other Notes

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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This Item	D9780	D2916	D0672
Sigma-Aldrich D9281 Lambda DNA EcoR I Hind III Digest	Sigma-Aldrich D9780 Lambda DNA Hind III Digest	Sigma-Aldrich D2916 Lambda DNA Mixed Digest	Sigma-Aldrich D0672 φX174 DNA Marker Hae III Digest
Quality Level 100, 200	Quality Level 200	Quality Level 100, 200	Quality Level 100, 200
form liquid	form liquid	form buffered aqueous solution	form liquid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)
usage 100 uses (0.1 mg size)	usage 100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)	usage 100 uses (0.1 mg size), 20 uses (20 μg size)	usage 200 uses (0.1 mg size), 40 uses (20 μg size)