The kit does not have specified lower and upper detection limits for enzyme activity. The enzyme activity needs to be empirically determined using varying enzyme amounts.
CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Synonym(s):
Beta-NAG Activity Assay Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
Pricing and availability is not currently available.
Quality Segment
usage
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NAGLU(4669)
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
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This Item | |||
|---|---|---|---|
| Gene Information human ... NAGLU(4669) | Gene Information human ... CS(1431) | Gene Information human ... HMGCR(3156) | Gene Information human ... ALPL(249) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL) | usage kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates) | usage sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL) | usage kit sufficient for 300 reactions (200 μl reactions) |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
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Kit Components Only
Product No.
Description
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
target_organs
Liver,Kidney
wgk
WGK 3
Storage Class
10 - Combustible liquids
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CS0780-1KT | 04061833553145 |
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What are the lower and upper limits of enzyme activity that the CS0780 B-N-Acetylglucosaminidase Assay Kit can measure?
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