Currently, there are no Bromelain products tested with casein and reported in CDU.
B5144
Bromelain from pineapple stem
≥4 units/mg protein, (chromatography purified)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
Specific activity:
(chromatography purified), ≥4 units/mg protein
Pricing and availability is not currently available.
form
lyophilized powder
Quality Level
specific activity
(chromatography purified), ≥4 units/mg protein
composition
Protein, 30-50% biuret
solubility
H2O: soluble 1.0 mg/mL, clear, colorless
storage temp.
−20°C
Application
Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Physical form
Lyophilized powder containing mannitol and potassium phosphate buffer salts
Preparation Note
Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.
Other Notes
One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity (chromatography purified), ≥4 units/mg protein | specific activity ≥3 units/mg protein | specific activity ≥100 units/mg protein | specific activity ≥3 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: soluble 1.0 mg/mL, clear, colorless | solubility - | solubility - | solubility H2O: soluble 0.5 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| composition Protein, 30-50% biuret | composition Protein, ≥30% biuret | composition Protein, 20-74% Lowry | composition Protein, ~70% biuret |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B5144-250UN | 04061833433591 |
| B5144-100UN | 04061833433584 |
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Good day! I would like to ask if do you have a Bromelain Powder in CDU? We would like to purchase for our research regarding bromelain enzyme.
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