While the total molecular weight of aldolase is reported to be 160 kDa, this enzyme has not been tested for use as a molecular weight marker. Suitability would have to be determined by the end users.
A2714
Aldolase from rabbit muscle
lyophilized powder, ≥8.0 units/mg protein
Synonym(s):
D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-781-0
MDL number:
Specific activity:
≥8.0 units/mg protein
Biological source:
rabbit muscle
Pricing and availability is not currently available.
biological source
rabbit muscle
Quality Segment
form
lyophilized powder
specific activity
≥8.0 units/mg protein
composition
Protein, ≥80% biuret
foreign activity
glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%
storage temp.
−20°C
Application
Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [1]. Product A2714 is essentially sulfate-free and contains citrate buffer salts.
Biochem/physiol Actions
Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold [1].
Physical form
Essentially sulfate-free containing citrate buffer salts
Other Notes
One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.
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This Item | |||
|---|---|---|---|
| specific activity ≥8.0 units/mg protein | specific activity 10-20 units/mg protein | specific activity ≥100 units/mg protein | specific activity ≥4,000 units/mg protein |
| biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle |
| form lyophilized powder | form ammonium sulfate suspension | form ammonium sulfate suspension | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, triosephosphate isomerase ≤0.05%, lactic dehydrogenase ≤0.03%, pyruvate kinase ≤0.1% | foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05% | foreign activity lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05% | foreign activity 3-phosphoglyceric phosphokinase ≤0.01%, glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001% |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A2714-200UN | 04061833359679 |
| A2714-500UN | 04061833627587 |
| A2714-100UN | 04061833359662 |
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Can this product be used as the protein molecular weight standard (representing 160kDa) in the SEC chromatography analysis?
1 answer-
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What is the molecular weight (MW) of the product?
1 answer-
The molecular weight of aldolase from rabbit muscle is 161,000 daltons, and it is a tetramer composed of subunits of 40,000 daltons. This information is detailed in the research article: "The molecular weight of rabbit muscle aldolase and the properties of the subunits in acid solution" by Sia, C.L., and Horecker, B.L., published in the Arch. Biochem. Biophys. journal, volume 123, issue 1, pages 186-194 in 1968.
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