Fat or lipid dyes, such as the Sudan and Nile series, typically stain by dissolving the dye into a solvent with low solubility, followed by further dilution with water for use in most staining protocols. When the dye encounters a fat or lipid, it moves from the solvent with low solubility to the solvent with higher solubility. Nile Red is soluble in DMF, DMSO, heptane, xylene, 1% in acetone, and 0.1% in ethanol. There is no specific procedures for staining nanoparticles. However, if the nanoparticles contain a fat or lipid component, Nile Red is expected to work. As for the particle size in solution, there is no available information.
72485
Nile Red
suitable for microscopy
Nile Red
Synonym(s):
Nile Blue A Oxazone
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About This Item
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
230-966-0
Beilstein/REAXYS Number:
279110
MDL number:
Pricing and availability is not currently available.
form
crystals
Quality Segment
mol wt
318.37 g/mol
color
brown to very dark brown, red to very dark red
mp
203-205 °C (lit.)
solubility
methanol: 0.01 g/10 mL (red to very dark red)
λmax
553 nm
suitability
suitable for microscopy
SMILES string
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
General description
Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.
Application
Nile Red has been used:
- as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
- to label nanoparticles (NPs)
- for lipid analysis
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This Item | |||
|---|---|---|---|
| suitability suitable for microscopy | suitability suitable for fluorescence | suitability - | suitability - |
| color brown to very dark brown, red to very dark red | color - | color - | color - |
| form crystals | form solid | form powder | form powder |
| solubility methanol: 0.01 g/10 mL (red to very dark red) | solubility - | solubility methanol: 1 mg/mL | solubility H2O: 1 mg/mL, blue |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| mp 203-205 °C (lit.) | mp 203-205 °C (lit.), 205-215 °C | mp 203-205 °C (lit.) | mp >300 °C (dec.) (lit.) |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can Nile Red be prepared for staining nanoparticles, and what is the process for staining different particle sizes?
1 answer-
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Can the crystal form of Nile Red be used in the same way as the powder form, specifically in terms of using acetone to dissolve and create working solutions?
1 answer-
The form of the dye, whether as a powder or crystals, should not impact the process of reconstituting Nile Red or most other dyes. For product 72485, both the powder and crystal forms have the same solubility specification. Therefore, either form is acceptable. It's advisable to first use the solvent recommended in a published procedure if following one.
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