This product has not been tested for use in any specific application. The material is soluble in DMSO, Dimethylformamide, and water. Please see the link below to review additional publications that may be helpful:
https://www.sciencedirect.com/topics/chemistry/ethidium-homodimer
46043
Ethidium homodimer
suitable for fluorescence, ~90% (HPCE)
Synonym(s):
EtDi, EthD-1
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About This Item
Empirical Formula (Hill Notation):
C46H50Cl4N8
CAS Number:
Molecular Weight:
856.75
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12161505
EC Number:
263-325-9
MDL number:
Pricing and availability is not currently available.
Quality Level
description
suitable for the fluorogenic detection of DNA
assay
~90% (HPCE)
form
solid
mol wt
856.75 g/mol
concentration
≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride)
color
red to dark red
mp
≥250 °C (lit.)
solubility
DMF: soluble, DMSO: soluble, H2O: soluble
fluorescence
λex 493 nm in H2O, λex 528 nm; λem 617 nm in TE buffer; DNA
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
InChI
1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
InChI key
ZKAJUKABUUJRLB-UHFFFAOYSA-N
Application
Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Reagent for the fluorimetric detection of nucleic acids[1]
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 493 nm in H2O, λex 528 nm; λem 617 nm in TE buffer; DNA | fluorescence - | fluorescence - | fluorescence - |
| color red to dark red | color red | color - | color - |
| concentration ≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride) | concentration 2 mM in DMSO | concentration - | concentration - |
| form solid | form liquid | form liquid | form powder |
| solubility DMF: soluble, H2O: soluble, DMSO: soluble | solubility - | solubility - | solubility - |
signalword
Danger
hcodes
Storage Class
4.3 - Hazardous materials which set free flammable gases upon contact with water
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Hazard Classifications
Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react. 3
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Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids.
J Markovits et al.
Analytical biochemistry, 94(2), 259-264 (1979-04-15)
Susanne Radonjic-Hoesli et al.
The Journal of allergy and clinical immunology, 140(6), 1632-1642 (2017-04-17)
Eosinophils are a subset of granulocytes that can be involved in the pathogenesis of different diseases, including allergy. Their effector functions are closely linked to their cytotoxic granule proteins. Release takes place through several different mechanisms, one of which is
Nai-Chen Cheng et al.
Acta biomaterialia, 51, 258-267 (2017-01-31)
Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 46043-5MG-F | 04061833277232 |
| 46043-1MG-F | 04061832343891 |
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