2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
35848
2′,7′-Dichlorofluorescein
BioReagent, suitable for fluorescence, ≥90% (T)
Synonym(s):
DCF, Dichlorofluorescein
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About This Item
Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
Molecular Weight:
401.20
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
200-968-6
Beilstein/REAXYS Number:
58009
MDL number:
Pricing and availability is not currently available.
product line
BioReagent
Quality Level
assay
≥90% (T)
form
solid
mp
280 °C (dec.) (lit.)
fluorescence
λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. [1] The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[2]
Application
2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. [3] It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.
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This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability suitable for fluorescence |
| fluorescence λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 | fluorescence - | fluorescence - | fluorescence λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) |
| form solid | form powder | form powder | form powder or crystals |
| assay ≥90% (T) | assay ~90% (TLC) | assay - | assay ≥95% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| mp 280 °C (dec.) (lit.) | mp 280 °C (dec.) (lit.) | mp 280 °C (dec.) (lit.) | mp 232-234 °C (lit.) |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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H. Watanabe et al.
Analytical Sciences, 2, 461-461 (1986)
W.D. Pfeffer et al.
Journal of Chromatography A, 506, 401-401 (1990)
Xiuping Chen et al.
Free radical research, 44(6), 587-604 (2010-04-08)
Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 35848-25G | 04061831812831 |
| 35848-5G | 04061831812848 |
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What can be used to make a solution of 2´,7´-dichlorofluorescein?
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
Helpful?
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In what types of applications can 2´,7´-Dichlorofluorescein be used?
1 answer-
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC. See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
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