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Atto 647 NHS ester

Name: Atto 647 NHS ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

UNSPSC Code:

12352108

NACRES:

NA.25

MDL number:

MFCD05865409

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Properties

product line

BioReagent

Quality Segment

100

assay

≥90% (HPLC), ≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 646-652 nm A_max

suitability

suitable for fluorescence

storage temp.

−20°C

Description

Application

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye. Atto 647 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, good water solubility, and very little triplet formation. Atto 647 is a zwitterionic dye with a net electrical charge of zero. Atto 647 is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH.

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item	01464	00379	08741
Sigma-Aldrich 07376 Atto 647 NHS ester	Supelco 01464 Atto 633 NHS ester	Sigma-Aldrich 00379 Atto 495 NHS ester	Supelco 08741 Atto 594 NHS ester
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
product line BioReagent	product line BioReagent	product line BioReagent	product line BioReagent
UV absorption λ: 646-652 nm A_max	UV absorption λ: 627-633 nm A_max	UV absorption λ: 496-502 nm A_max	UV absorption λ: 599-605 nm A_max

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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