It is not feasible to dissolve catalog number E4009 to a concentration of 1 to 5% in alcohol. For H&E staining or staining sperm for viability, Eosin Y disodium salt is typically used, rather than Eosin Y free acid. Dry dyes, available under catalog numbers E4382 or 861006, are also used, along with various solutions for H&E staining, which also utilize only Eosin Y disodium salt. A 5% Eosin Y solution is available with the catalog number 318906, which is also formulated with Eosin Y disodium salt.
E4009
Eosin Y
Dye content ~99%, Powder
Synonym(s):
2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43
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About This Item
Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
Molecular Weight:
647.89
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
239-138-3
MDL number:
Colour Index Number:
45380:2
Pricing and availability is not currently available.
Product Name
Eosin Y, Dye content ~99 %
form
powder
Quality Segment
composition
Dye content, ~99%
color
light red to red/pink
solubility
ethanol: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br
InChI
1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H
InChI key
DBZJJPROPLPMSN-UHFFFAOYSA-N
Application
Eosin Y has been used as a stain:
- to determine the location of the brain sections
- for staining of fixed liver tissue
- for histological examination of brain sections from the dorsal hippocampus
Biochem/physiol Actions
Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.
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This Item | |||
|---|---|---|---|
| color light red to red/pink | color brown | color brown | color orange to very dark orange |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility ethanol: 1 mg/mL | solubility H2O: 1 mg/mL | solubility H2O: 1 mg/mL | solubility spirit: soluble |
| form powder | form powder | form powder | form powder |
| composition Dye content, ~99% | composition , ≥85% HPLC | composition Dye content, ≥90% | composition - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is there a sample protocol for this product ? There id low solubility of E4009 in even ethanol (1 mg/mL) in that case how to make the usual eosin concentration of 1-5% w/v for use in an H&E staining reaction.
1 answer-
Helpful?
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What is the purity level for product E4009 and can it be added to CoA?
1 answer-
t is not possible to add a purity specification to Eosin Y free acid because no purity tests are performed
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