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D5545

DL-Dithiothreitol

BioXtra, ≥99.0% (titration)

Synonym(s):

threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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About This Item

Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
3483-12-3
Molecular Weight:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
24893941
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
MFCD00004877
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product line

BioXtra

Quality Segment

200

assay

≥99.0% (titration)

form

powder

reaction suitability

reagent type: reductant

impurities

Insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane

pH

4.0-6.0 (20-25 °C, 0.1 m in H2O)

mp

41-44 °C (lit.)

solubility

H2O: 0.1 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.1%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.100 at 280 in H2O at 0.1 M, ≤0.400 at 260 in H2O at 0.1 M

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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This Item
D9779D916343815
DL-Dithiothreitol BioXtra, ≥99.0% (titration)

Sigma-Aldrich

D5545

DL-Dithiothreitol

DL-Dithiothreitol Molecular Biology, ≥98% (HPLC), ≥99% (titration)

Sigma-Aldrich

D9779

DL-Dithiothreitol

DL-Dithiothreitol suitable for electrophoresis, ≥99%

Sigma-Aldrich

D9163

DL-Dithiothreitol

DL-Dithiothreitol BioUltra, Molecular Biology, ≥99.5% (RT)

Sigma-Aldrich

43815

DL-Dithiothreitol

assay

≥99.0% (titration)

assay

≥98% (HPLC), ≥99% (titration)

assay

≥99%

assay

≥99.5% (RT)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioXtra

product line

-

product line

-

product line

BioUltra

anion traces

sulfate (SO42-): ≤0.005%

anion traces

-

anion traces

-

anion traces

sulfate (SO42-): ≤50 mg/kg

mp

41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

form

powder

form

powder

form

powder

form

powder

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pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000557072
0000557073
0000557074
0000554577
0000554578

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Global Trade Item Number

SKUGTIN
D5545-25G04061835544011
D5545-5G04061835515981
D5545-1G04061833586419

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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