This compound is soluble in Dimethylformamide:methanol (1:1) at 50 mg/mL, Dimethylformamide at 20 mg/mL, and DMSO at 20 mg/mL. When protected from light, stock solutions of X-gal in the given solvents are stable for up to 2 months at 2 to 8 °C and up to 1 year in aliquots at -20 °C.
B4252
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
chromogenic, ≥98%, powder
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
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About This Item
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
NACRES:
NA.83
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1402009
UNSPSC Code:
12352204
EC Number:
230-640-8
Pricing and availability is not currently available.
Product Name
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder
Quality Level
assay
≥98%
form
powder
solubility
DMF: methanol (1:1): 50 mg/mL
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key
OPIFSICVWOWJMJ-AEOCFKNESA-N
Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
1 of 1
This Item | |||
|---|---|---|---|
| assay ≥98% | assay ≥99% (HPLC) | assay 99% | assay - |
| solubility DMF: methanol (1:1): 50 mg/mL | solubility DMSO: 2% (20mg/mL) | solubility DMF: soluble, methanol: soluble | solubility - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
| form powder | form solid | form solid | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B4252-2G | 04061833616550 |
| B4252-500MG | 04061833429419 |
| B4252-5G | 04061833429426 |
| B4252-100MG | 04061835540693 |
| B4252-50MG | 04061835553808 |
| B4252-1G | 04061835480234 |
| B4252-250MG | 04061835553792 |
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Could you Please provide information on the solvent used for item B4252-50MG ?
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