90107

Gram′s iodine solution

suitable for microscopy

• Synonym(s): Potassium Triiodide
• NACRES: MA.02
• UNSPSC Code: 12171500

Properties

• product line: BioChemika
• Quality Segment: 100
• form: liquid
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• color: brown to very dark brown
• refractive index: n20/D 1.335
• density: 1.005 g/mL at 20 °C
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• suitability: suitable for microscopy, bacteria

Description

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Safety Information

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves
• pictograms: GHS08
• signalword: Danger
• hcodes: H360D
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects