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Urea

Name: Urea
Brand: SIAL

ACS reagent grade, ≥99%, solid, puriss. p.a., reag. Ph. Eur.

Synonym(s):

Carbamide, Carbonyldiamide

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About This Item

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

NACRES:

NA.21

PubChem Substance ID:

57648752

UNSPSC Code:

12352100

EC Number:

200-315-5

MDL number:

MFCD00008022

Beilstein/REAXYS Number:

635724

Assay:

≥99%, 99-101% (calc. on dry substance)

Form:

solid

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Properties

Product Name

Urea, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

Quality Level

200

agency

reag. Ph. Eur.

grade

ACS reagent, puriss. p.a.

assay

≥99%, 99-101% (calc. on dry substance)

form

solid

impurities

alkalic reac. substances, complies, ≤0.0005% heavy metals (as Pb), ≤0.05% ammonium (NH₄), ≤0.1% biuret

ign. residue

≤0.01% (as SO₄)

loss

≤1.0% loss on drying, 105°C, 1 h

mp

132-135 °C

solubility

H₂O: soluble 480 g/L at 20 °C

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤5 mg/kg, sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Fe: ≤2 mg/kg

suitability

in accordance for appearance of solution, in accordance for identity

functional group

amine

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

Application

Urea has been used in the following studies:

Synthesis of SnO₂ nanoparticles.[1]
Synthesis of urethanes, ureides and semicarbazides.
Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.[2]
Protein electrophoretic studies.[3]

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

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This Item	U5128	U1250	15604
Sigma-Aldrich 33247 Urea	Sigma-Aldrich U5128 Urea	Sigma-Aldrich U1250 Urea	Sigma-Aldrich 15604 Urea
assay ≥99%, 99-101% (calc. on dry substance)	assay ≤0.001% (Heavy Metals by ICP-OES, metals basis), 99.0-100.5%	assay ≥99.5%	assay 99.0-100.5%, 99.0-101.0% (calc. on dry substance)
solubility H₂O: soluble 480 g/L at 20 °C	solubility H₂O: soluble 480 g/L at 20 °C	solubility H₂O: 8 M	solubility H₂O: soluble 480 g/L at 20 °C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
mp 132-135 °C	mp 132-135 °C (lit.)	mp 132-135 °C (lit.)	mp 132-135 °C
form solid	form solid	form pellets	form solid
density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)	density 1.335 g/mL at 25 °C (lit.)

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The Fertilizer Encyclopedia, 709-709 (2009)

Performance characteristics of a new hybrid quadrupole time-of-flight tandem mass spectrometer (TripleTOF 5600).

Genna L Andrews et al.

Analytical chemistry, 83(13), 5442-5446 (2011-05-31)

The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. Employing a Saccharomyces

Proteomic analysis of exosomes secreted by human mesothelioma cells.

Joost P J J Hegmans et al.

The American journal of pathology, 164(5), 1807-1815 (2004-04-28)

Exosomes are small membrane vesicles secreted into the extracellular compartment by exocytosis. Tumor exosomes may be involved in the sampling of antigens to antigen presenting cells or as decoys allowing the tumor to escape immune-directed destruction. The proteins present in

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Global Trade Item Number

SKU	GTIN
33247-5KG	04061826736760
33247-2.5KG	04061832977560
33247-1KG	04061835365654
33247-250G	04061826736753

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