11051482001

dTTP

Lithium salt

• Synonym(s): Thymidine 5′-triphosphate sodium salt, 2′-deoxy-thymidine-5′-triphosphate, deoxy-thymidine-5′-triphosphate, 2′-, TTP, dTTP
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₁₄P₃Na
• CAS Number: 18423-43-3
• Molecular Weight: 504.15
• UNSPSC Code: 41116100
• PubChem Substance ID: 329749302
• MDL number: MFCD00080338
• NACRES: NA.54
• Assay: ≥98%
• Form: solution
• Storage temp.: −20°C

Properties

• assay: ≥98%
• form: solution
• usage: sufficient for 6,250 standard reactions (PCR)
• packaging: pkg of 250 μL (25 μmol)
• manufacturer/tradename: Roche
• concentration: 100 mM
• technique(s): DNA amplification: suitable, DNA sequencing: suitable
• storage temp.: −20°C
• SMILES string: [NaH].[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)C)O)(O[P](=O)(O)O)O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChI key: KAELIXTVSANDSE-UHFFFAOYSA-N

Description

General description

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

Application

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting[1][2] and PCR techniques.[3]

Analysis Note

Typical analysis:

• ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Other Notes

1 μmol dTTP = 9.5 A₂₆₀units

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: No data available
• flash_point_c: No data available