The protocol states to ensure the effects of the buffer are taken into consideration, it is recommended that the dilutions are made in the same buffer as the samples. Any interference from the buffer can then be corrected for in the BSA standard curve. The working reagent is prepared by mixing 50 parts BCA solution with 1 part of 4% Cupric Sulfate. The suitability of the standard dilutions over time has not been evaluated. To ensure accurate results and optimum performance, fresh standard curves should be prepared.
71285-M
BCA Protein Assay Kit
The BCA protein assay is a simple and reliable protein quantification method.
BCA Protein Assay Kit
Synonym(s):
BCA Protein Quantification, Bicinchoninic Acid Assay, Protein Assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
41106500
Pricing and availability is not currently available.
manufacturer/tradename
Novagen®
storage condition
OK to freeze
storage temp.
10-30°C
General description
The BCA protein assay is a simple and reliable protein quantification method.
The BCA protein assay is based on a biuretreaction, which is the reduction of Cu2+ toCu+ by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. Bicinchoninic acidis a chromogenic reagent that chelates thereduced copper, producing a purple complexwith strong absorbance at 562 nm (Smith1985, Wiechelman 1988). This assay can beused to quantify protein concentration with awide variety of samples and can be performedin minutes.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
The Novagen® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 µl proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 µl protein sample plus 200 µlreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster Protein Extraction Reagent,PopCulture Reagent, CytoBuster ProteinExtraction Reagent, Reportasol ExtractionBuffer, and Insect PopCulture Reagent. Optionsfor the removal or dilution of interferingsubstances are described in the kit literature.
Other Notes
•500 mlBCA Solution
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
Smith, P.K., et al. 1985. Anal. Biochem.150, 76.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.
Wiechelman, K., et al. 1988. Anal. Biochem.175, 231.
Brown, R., et al. 1989. Anal. Biochem.180, 136.
Legal Information
Use of the BCA protein assay is permitted for research purposes only.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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This Item | |||
|---|---|---|---|
| manufacturer/tradename Novagen® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| storage temp. 10-30°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 71285-3 | 07790788060435 |
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Good morning, can the standard's dilutions be reutilized for different days or should be discarted after one use? Should we add a standard curve every time that we want to measure proteins?
1 answer-
Helpful?
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Can this work to determine the amount of Human Serum Albumin or it's only working for BSA?
1 answer-
Yes, this kit will determine the protein content of a given sample, including Human Serum Albumin (HSA). If desired, HSA may be substituted in place of BSA, in generating a standard curve.
Helpful?
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Hallo, ist das Assay mit DMEM Medium und Phenol Rot kompatibel?
1 answer-
Phenol Red has been found to interfere with this kit. DMEM compatibility will depend on its composition. Please see below for identified interfering substances. Additional information is available in the Substance Compatibility section of the product user guide, pages 5 - 7, at the link below:
https://www.emdmillipore.com/US/en/product/BCA-Protein-Assay-Kit,EMD_BIO-71285#anchor_USPHelpful?
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