The approximate mass of this product will depend on the specific activity. According to the product specifications, the activity is ≥250 units/mg solid, and the product is supplied in a 10 KU pack size. For a lot with 250 units/mg, the approximate mass will be 40 mg. Please check the certificate of analysis for the actual activity of each lot. This recombinant protein does not contain any epitope tag for purification purposes.
345386
Glucose Oxidase, Aspergillus niger, Recombinant
Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.
Synonym(s):
Glucose Oxidase, Aspergillus niger, Recombinant
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About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥250 units/mg solid
Pricing and availability is not currently available.
Quality Level
description
Merck USA index - 14, 4460
form
lyophilized solid
specific activity
≥250 units/mg solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
yellow-brown
solubility
0.1 M phosphate buffer, pH 7.0: 5 mg/mL
foreign activity
Amylase ≤0.1%, catalase ≤20 U/mg (dry weight), sucrase ≤0.1%
shipped in
ambient
storage temp.
2-8°C
SMILES string
O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O
InChI
1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
InChI key
WQZGKKKJIJFFOK-VFUOTHLCSA-N
General description
Note: 1 KU = 1000 units.
Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.
Preparation Note
Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.
Other Notes
Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.
One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| specific activity ≥250 units/mg solid | specific activity ≥100,000 units/g solid (without added oxygen) | specific activity ≥10,000 units/g solid (without added oxygen) | specific activity 100,000-250,000 units/g solid (without added oxygen) |
| form lyophilized solid | form lyophilized powder | form powder | form lyophilized powder |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
| shipped in ambient | shipped in wet ice | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility 0.1 M phosphate buffer, pH 7.0: 5 mg/mL | solubility - | solubility - | solubility - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hema Chandra Kotamarthi et al.
Cell reports, 30(8), 2644-2654 (2020-02-27)
ATP-powered unfoldases containing D1 and D2 AAA+ rings play important roles in protein homeostasis, but uncertainty about the function of each ring remains. Here we use single-molecule optical tweezers to assay mechanical unfolding and translocation by a variant of the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 345386-10KU | 07790788049300 |
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Hello! We are looking at recombinant glucose oxidase from Aspergillus Niger, 345386. Does this contain a His-tag for purification? Also could you please provide an estimate for the mass, based on a given activity. What is the approximate mass of 10 KU?
1 answer-
Helpful?
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what is the application of Recombinant Glucose Oxidase sigma? can use it in medical?
1 answer-
This product is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors. It is useful for enzymatic determination of glucose concentrations in various samples. Unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.
It may be helpful to review the following publications below:
https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2165
https://academic.oup.com/lambio/article-abstract/58/4/393/6699633?redirectedFrom=fulltextHelpful?
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