E6632
Epoxy-activated-Agarose
lyophilized powder
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About This Item
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form
lyophilized powder
Quality Level
extent of labeling
≥20 μmol per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix active group
epoxy
matrix attachment
through epoxy to hydroxyl
matrix spacer
12 atoms
swelling
1 g swells to ~3 mL
suitability
suitable for chromatography
storage temp.
−20°C
General description
when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
Epoxy-activated-Agarose is a resin. Ligands can be covalently linked to agarose via the interaction of the nucleophilic groups of the ligand with the epoxy group.
Application
Epoxy-activated-Agarose is a lyophilized powder, stabilized with lactose, which is used in affinity chromatography, protein chromatography and activated/functionalized matrices. Epoxy-activated agarose has been used in studies informing anti-proliferative activity on human-derived cancer cells as well as cancer prevention.
Physical form
Lyophilized powder stabilized with lactose
Other Notes
Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.
1 of 1
This Item | |||
|---|---|---|---|
| matrix cross-linked 4% beaded agarose | matrix 6% agarose | matrix - | matrix - |
| matrix active group epoxy | matrix active group - | matrix active group - | matrix active group - |
| form lyophilized powder | form - | form powder | form solid |
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 20-25°C | storage temp. - |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Adel M Nour et al.
Infection and immunity, 77(3), 1262-1271 (2009-01-07)
Multiple microbial components trigger the formation of an inflammasome complex that contains pathogen-specific nucleotide oligomerization and binding domain (NOD)-like receptors (NLRs), caspase-1, and in some cases the scaffolding protein ASC. The NLR protein Nalp1b has been linked to anthrax lethal
Donald Voet and Judith G. Voet
Biochemistry (2004)
G J Fennington et al.
Microbiology (Reading, England), 142 ( Pt 2), 321-330 (1996-02-01)
The Rhizobium leguminosarum bv. trifolii BAL fructokinase (frk) gene was isolated on a 2 center dot 4 kb BamHI fragment from the cosmid pLA72 by complementation analysis of the Tn5-induced frk mutant BAL79, and confirmed by hybridization analysis. The nucleotide
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E6632-1G | 04061826690826 |
| E6632-5G | 04061833428146 |