S3147

5-Sulfosalicylic acid dihydrate

suitable for electrophoresis, ≥99%

• Synonym(s): 2-Hydroxy-5-sulfobenzoic acid
• Linear Formula: HO₃SC₆H₃-2-(OH)CO₂H·2H₂O
• CAS Number: 5965-83-3
• Molecular Weight: 254.21
• UNSPSC Code: 41105319
• NACRES: NB.22
• PubChem Substance ID: 24899547
• EC Number: 202-555-6
• Beilstein/REAXYS Number: 650741
• MDL number: MFCD00149540

Properties

• Quality Segment: 200
• assay: ≥99%
• form: powder or crystals
• technique(s): electrophoresis: suitable
• impurities: ≤0.02% Insoluble matter, ≤0.05% Salicylic Acid
• mp: 105-110 °C (lit.)
• solubility: water: soluble 100 mg/mL, clear, colorless
• anion traces: chloride (Cl^-): ≤0.001%, sulfate (SO₄^2-): ≤0.035%
• cation traces: Fe: ≤0.001%, heavy metals (as Pb): ≤0.002%
• SMILES string: [H]O[H].[H]O[H].OC(=O)c1cc(ccc1O)S(O)(=O)=O
• InChI: 1S/C7H6O6S.2H2O/c8-6-2-1-4(14(11,12)13)3-5(6)7(9)10;;/h1-3,8H,(H,9,10)(H,11,12,13);2*1H2
• InChI key: BHDKTFQBRFWJKR-UHFFFAOYSA-N

Description

General description

5-Sulfosalicylic acid is a strong acid capable of protonating water. It forms a new theophylline complex, which was investigated by X-ray photoelectron spectroscopy (XPS).[1] It forms 1:1 proton-transfer compounds with the ortho-substituted monocyclic heteroaromatic Lewis bases. Their crystal structures have been studied.

Application

5-Sulfosalicylic acid dihydrate may be employed for the quantification of doxorubicin derived from PEGylated liposomal doxorubicin (Doxil) and its major metabolite in human plasma by HPLC.[2] It may be used in the preparation of poly[[tris(di-2-pyridylamine)tris([μ]-5-sulfonatosalicylato)tricopper(II)] trihydrate].

5-Sulfosalicylic acid (3.5%) in 12% trichloroacetic acid is commonly used as a fixing solution for proteins in agarose and polyacrylamide gels.

Analysis Note

Tested for use in fixing solutions for PAGE, SDS-PAGE and IEF.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H314
• pcodes: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves