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  • The potential of DBP gels containing intervertebral disc cells for annulus fibrosus supplementation: in vivo.

The potential of DBP gels containing intervertebral disc cells for annulus fibrosus supplementation: in vivo.

Journal of tissue engineering and regenerative medicine (2013-03-06)
Jeong Eun Song, Eun Young Kim, Woo Young Ahn, Yu Jeong Lee, Dongwon Lee, Rui Reis, Gilson Khang
要旨

Demineralized bone particle (DBP), which is widely used as a biomaterial in the field of tissue engineering, contains various bioactive molecules, such as cytokines. For this reason, in this study we investigated the effects of injectable DBP gels on cell proliferation, inflammation and maintenance of the shape of DBP gels as a scaffold able to substitute for intervertebral discs (IVDs) in vivo. DBP gels were fabricated with different percentages (5% and 10%) of DBP powder and 3% acetic acid, including 0.02% pepsin. DBP gels with 1 × 10(6) annulus fibrosus (AF) cells were implanted into the dorsal subcutaneous region of BALB/C-nu mice for 1, 2 and 3 weeks. Cell proliferation was measured by MTT assay. The effect of DBP gels on the inflammatory response was analysed by measuring the amount of tumour necrosis factor-alpha (TNFα) released. Also, histological methods were carried out to analyse the response of DBP gels in vivo. This study demonstrated that injectable DBP gels are able to provide physical scaffolds for growing IVD cells in vivo.

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製品内容

Sigma-Aldrich
ジメチルスルホキシド, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
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ジメチルスルホキシド, ACS reagent, ≥99.9%
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ジメチルスルホキシド, for molecular biology
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塩酸, ACS reagent, 37%
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酢酸, glacial, ACS reagent, ≥99.7%
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ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
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酢酸, glacial, ReagentPlus®, ≥99%
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塩酸, ACS reagent, 37%
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リン酸ナトリウム 二塩基性, ACS reagent, ≥99.0%
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ジメチルスルホキシド, ReagentPlus®, ≥99.5%
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塩化水素 溶液, 4.0 M in dioxane
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ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture
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チアゾリルブルーテトラゾリウムブロミド, 98%
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ジメチルスルホキシド, puriss. p.a., ACS reagent, ≥99.9% (GC)
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酢酸, glacial, ≥99.99% trace metals basis
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酢酸, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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