At physiological pH, phenol red strongly absorbs in the same wavelength range as the MTT assay’s readout product. Washing cells thoroughly to remove the media and phenol red minimizes this interference.
M5655
チアゾリルブルーテトラゾリウムブロミド
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
別名:
3-(4,5-ジメチル-2-チアゾリル)-2,5-ジフェニル-2H-テトラゾリウムブロミド, MTT, メチルチアゾリルジフェニル-テトラゾリウムブロミド
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サイズを選択してください
100 MG
￥10,900
500 MG
￥24,400
1 G
￥36,400
5 X 1 G
￥122,000
￥10,900
出荷可能日2025年4月07日詳細
すべての画像(5)
サイズを選択してください
表示を変更する
100 MG
￥10,900
500 MG
￥24,400
1 G
￥36,400
5 X 1 G
￥122,000
About This Item
実験式（ヒル表記法）:
C18H16BrN5S
CAS番号:
分子量:
414.32
Beilstein:
4081397
EC Number:
MDL番号:
UNSPSCコード:
12352207
PubChem Substance ID:
NACRES:
NA.75
￥10,900
出荷可能日2025年4月07日詳細
おすすめの製品
製品種目
BioReagent
品質水準
アッセイ
≥97.5% (HPLC)
フォーム
powder
テクニック
cell culture | insect: suitable
cell culture | mammalian: suitable
mp
195 °C (dec.) (lit.)
溶解性
H2O: 5 mg/mL
保管温度
2-8°C
SMILES記法
[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
InChI
1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI Key
AZKSAVLVSZKNRD-UHFFFAOYSA-M
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関連するカテゴリー
アプリケーション
チアゾリルブルーテトラゾリウムブルー（MTT）を細胞増殖の測定に使用する場合があります。 MTTは黄色の溶液を生じますが、これは生細胞のミトコンドリアデヒドロゲナーゼにより濃青色の水不溶性MTTホルマザンに変換されます。青色の結晶は酸性化イソプロパノールで可溶化され、570 nmで比色的に強度を測定します。
チアゾリルブルーテトラゾリウムブルー（MTT）を細胞増殖の測定に使用する場合があります。 MTTは黄色の溶液を生じますが、これは生細胞のミトコンドリアデヒドロゲナーゼにより濃青色の水不溶性MTTホルマザンに変換されます。青色の結晶は酸性化イソプロパノールで可溶化され、570 nmで比色的に強度を測定します。MTTは、組織化学的試薬／細胞化学的試薬として、また、NADを検出するために使用されています。 組織中のADP結合酵素系は、使用されるシアニド（シアン化物）トラップによりカチオンが結合するため、MTTでは検出することができません。 MTTは急速にホルマザンに還元され、ホルマザンはニッケル、銅、およびコバルトでキレート化されます；コバルトキレートは酸化系で使用されています。
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
ターゲットの組織
Respiratory system
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
M5655-100MG:
M5655-BULK:
M5655-VAR:
M5655-500MG:
M5655-1G:
M5655-15MG:
M5655-15MG-KC:
M5655-5X1G:
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Why can't a medium with phenol red be used? Is it because it interferes with MTT assay results? If after incubation with MTT, cells are washed with PBS and DMSO is used to dissolve the crystals, does this still apply?
1 回答-
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Should I prepare fresh for each use or I directly resuspend in 20 ml of water (for the 100 mg bottle) and store? In the latter case, how can I store the solution?
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The solution may be filtered through a 0.2 μm filter and stored at 2-8°C for frequent use or stored in aliquots and frozen at -20°C for extended periods.
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What's the difference between M5655 and M2003?
1 回答-
Product M5655 is Thiazolyl Blue Tetrazolium Bromide and Product M2003 is Thiazolyl blue formazan.
MTT (Cat. no. M5655) is used in the measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan (Product M2003) by mitochondrial dehydrogenases of living cells. The blue MTT formazan crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Thus, the amount of blue formazan is proportional to the number of viable cells. For more information regarding this particular assay, we do offer an assay kit, TOX1, which is based on this principle: https://www.sigmaaldrich.com/product/sigma/tox1.
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What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?
1 回答-
We test the solubility of MTT in water at a concentration of 5 mg/mL. For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.
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Is the MTT assay quantitative?
1 回答-
The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
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How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?
1 回答-
Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source: Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.
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Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?
1 回答-
The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate. It also corrects for dust and fingerprints that can be present. The assay is read at 570 nm. The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.
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What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?
1 回答-
Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).
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How can I store solutions of Thiazolyl Blue Tetrazolium Bromide (MTT)?
1 回答-
Reconstituted MTT solution is stable for at least 6 months when stored frozen (-20 °C). Storage of reconstituted MTT solution at 2-8°C for more than 2 weeks may cause decomposition and yield erroneous results.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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