The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
￥41,500
出荷予定日2025年6月02日
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包装
pkg of 1 kit
テクニック
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
アプリケーション
cell analysis
detection
検出方法
colorimetric
輸送温度
dry ice
保管温度
−20°C
関連するカテゴリー
詳細
細胞成長判定キット（MTTベース）は、生細胞におけるミトコンドリア活性の関数として細胞増殖の分光光度測定ができるよう設計されています。MTT（3-(4,5-ジメチルチアゾイル-2)-2,5-ジフェニルテトラゾリウムブロミド）システムは、ミトコンドリアデヒドロゲナーゼ活性を介して生細胞の活性を測定する、単純で、正確で、再現性のある手段です。
アプリケーション
包装
5 mg/mL MTTを含有するRPMI(フェノ-ルレッドは含有しません)1 mL入りバイアル5本、50 mL MTT用溶媒(0.1 N HClを含有する無水イソプロパノ-ル)が梱包されています。キットの構成はマルチウェルプレ-トでの培養に適した量になっています。MTT溶液は全培養液量の10%で加えるため、1キットあたりの可能な試験回数は、培養容器や培養液量によって異なります。
関連製品
製品番号
詳細
価格
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
ターゲットの組織
Central nervous system
保管分類コード
3 - Flammable liquids
引火点(°F)
53.6 °F - closed cup
引火点(℃)
12 °C - closed cup
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
キットコンポーネントの情報を参照してください
PRTR
キットコンポーネントの情報を参照してください
消防法
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
キットコンポーネントの情報を参照してください
Jan Code
キットコンポーネントの情報を参照してください
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資料
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
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Is the MTT assay quantitative?
1 回答-
役に立ちましたか？
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What is the difference between Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 回答-
Product No. CGD1 and TOX1 are both MTT based assays. The major difference between Product No. CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
役に立ちましたか？
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What are the components of the Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 回答-
CGD1 contains 5 vials of 5 mg/vial of MTT and 50 ml of MTT solvent (solubilization solution) and TOX1 contains 5 vials of 15 mg/vial of MTT and 125 ml of solubilization solution.
役に立ちましたか？
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What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?
1 回答-
Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Can we use media with phenol red with Product CGD1, Cell Growth Determination Kit?
1 回答-
You most likely would not be able to use a medium containing phenol red with this kit. We have found that the phenol red can contribute to higher background, and therefore lower sensitivity.
役に立ちましたか？
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Is 10% fetal bovine serum (FBS) in the culture medium compatible with Product CGD1, Cell Growth Determination Kit?
1 回答-
High protein levels may form a precipitate when the MTT solubilization solution is added. Samples with protein concentrations equivalent to 10% FBS or 4 mg/mL protein seem acceptable.
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