We validated and added the IgG4-FITC antibody to our panel of DIF testing in July 2022. The pathologists are pleased with the results, and they find this antibody to serve as a sensitive marker for Bullous Pemphigoid.
F9890
Anti-Human IgG4−FITC antibody, Mouse monoclonal
clone HP-6025, purified from hybridoma cell culture
Anti-Human IgG4−FITC antibody, Mouse monoclonal
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Monoclonal Anti-Human IgG4
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
HP-6025, monoclonal
Application:
DIBA
Citations:
21
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
conjugate
FITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
secondary antibodies
clone
HP-6025, monoclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.
Application
Monoclonal Anti-Human IgG4-FITC antibody produced in mouse has been used in immunohistofluorescence and immunofluorescence assay.
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Biochem/physiol Actions
IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.
Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.
Physical form
Solution in 0.01 M phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4 | technique(s) dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32 | technique(s) ELISA: 1:80,000-1:160,000 using 1 μg/mL human IgG4 for coating | technique(s) indirect ELISA: 0.5 μg/mL using 1 μg/mL Human IgG1 myeloma protein for coating. |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| conjugate FITC conjugate | conjugate FITC conjugate | conjugate peroxidase conjugate | conjugate - |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype IgG2b |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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