Ugrás a tartalomra
Merck

XNAP

Sigma-Aldrich

REDExtract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Szinonimák:

Plant direct PCR kit, Plant direct PCR kit with loading dye

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106303
NACRES:
NA.55

használat

sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions

Minőségi szint

Jellemzők

dNTPs included
hotstart

technika/technikák

PCR: suitable

szín

red

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

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Általános leírás

The REDExtract-N-Amp Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Alkalmazás

REDExtract-N-Amp Plant PCR Kit has been used to:
  • extract genomic DNA from mycelial lawns and leaves
  • screen leaf tissue from putative transgenic potato plants
  • perform polymerase chain reaction (PCR)
  • isolate DNA from Cannabis sativa L. seed and dried fruit body gills

Tulajdonságok és előnyök

  • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
  • Compatible with high-throughput requirements for genetic analysis of plants
  • Extract stable at 4 °C for at least 6 months

Komponensek

Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Egyéb megjegyzések

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Jogi információk

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

kapcsolódó termék

Product No.
Leírás
Árazás

Piktogramok

Exclamation markEnvironment

Figyelmeztetés

Warning

Figyelmeztető mondatok

Veszélyességi osztályok

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Tárolási osztály kódja

10 - Combustible liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Rebeca Cobos et al.
Applied and environmental microbiology, 81(18), 6474-6483 (2015-07-15)
Grapevine trunk fungal pathogens, such as Diplodia seriata and Phaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of
Yolanda Arana-Gabriel et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(3), 632-640 (2018-02-28)
The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a
Giuseppe Forlani et al.
Forensic science international. Genetics, 52, 102464-102464 (2021-01-19)
The availability of molecular markers able to distinguish drug-type from fiber-type Cannabis sativa cultivars would allow fast and cheap analysis of any plant specimen, including seeds and leaves. Several approaches to this issue have been described, mainly using polymorphisms in
Samuel J Harrison et al.
Plant methods, 2, 19-19 (2006-11-08)
The floral dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium is the method of choice for Arabidopsis transformation. The presence of a marker, usually antibiotic- or herbicide-resistance, allows identification of transformed seedlings from untransformed seedlings.
Siobhan M Brady et al.
Molecular systems biology, 7, 459-459 (2011-01-20)
Tightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate

Cikkek

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Egyszerű DNS/RNS-tisztítási módszerek segítik a különböző forrásokból származó genomelemzést, növelve a kutatás hatékonyságát.

Protocols

REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.

Related Content

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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