T7039

Transforming Growth Factor-β1 human

≥98% (SDS-PAGE), recombinant, expressed in CHO cells, powder, suitable for cell culture

• Szinonimák: hTGF-β1, TGF-β1
• MDL-szám: MFCD00166089
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• product name: Transforming Growth Factor-β1 human, TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture
• biológiai forrás: human
• Minőségi szint: 200
• rekombináns: expressed in CHO cells
• Teszt: ≥98% (SDS-PAGE)
• form: powder
• hatékonyság: ≤0.05 ng/mL ED₅₀
• minőség: endotoxin tested
• molekulatömeg: protein 25 kDa
• kiszerelés: pkg of 2 μg; pkg of 50 μg
• tárolási körülmény: avoid repeated freeze/thaw cycles
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: <1 EU/μg
• UniProt elérési szám: P01137
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... TGFB1(7040)

Leírás

Általános leírás

Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.

Alkalmazás

Transforming Growth Factor-β1 human has been used in

• reporter gene assay.
• in vitro transfection.
• to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
• to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
• to study the interaction between TGF-β1 activation and myofibroblast formation.

Biokémiai/fiziológiai hatások

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Fizikai forma

Lyophilized from a 0.2 μm filtered aqueous solution.

Analízis megjegyzés

The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)