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T3809

Sigma-Aldrich

TRI Reagent®

BD, For processing whole blood, plasma, or serum.

Szinonimák:

DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® blood plasma serum, blood RNA extraction, blood RNA isolation, blood RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction reagent, total RNA extraction solution, total RNA isolation reagent, total RNA isolation solution, total RNA purification reagent, total RNA purification solution, TRI Reagent® RNA Isolation Reagent

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About This Item

MDL-szám:
MFCD00213058
UNSPSC kód:
12352200
NACRES:
NA.52
Az árazás és az elérhetőség jelenleg nem elérhető

Minőségi szint

200

használat

0.75 mL sufficient for 0.25 mL blood derivatives

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Általános leírás

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.
TRI Reagent® is a monophasic solution of phenol and guanidinium isothiocyanate.[1]

Alkalmazás

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
TRI Reagent® has been used for the isolation of RNA.[2][3]
The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

Tulajdonságok és előnyök

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Jogi információk

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Célzott szervek

Nervous system,Kidney,Liver,Skin

Egyéb veszélyek

EUH032

Tárolási osztály kódja

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Lobbanási pont (F)

174.2 °F - closed cup

Lobbanási pont (C)

79 °C - closed cup

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
MKCQ9520
MKCK7193
MKCH8720
MKCD4813
MKBX1805V

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COA keresése

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Dokumentumtár megtekintése

John-Edwin Thomson et al.
Pancreas, 48(1), 107-112 (2018-11-20)
Interleukin-21 (IL-21) is a cytokine associated with tissue inflammation, autoimmune and infectious diseases. Organ dysfunction and death can occur in patients with acute pancreatitis (AP) in two distinct clinical phases. Initially, a systemic inflammatory response syndrome may be followed by
A novel CLCN5 mutation in a Chinese boy with Dent's disease.
Ji LN
World Journal of Pediatrics : WJP, 10(3), 275-277 (2014)
Heiner Kuhl et al.
Molecular biology and evolution, 38(1), 108-127 (2020-08-12)
Presumably, due to a rapid early diversification, major parts of the higher-level phylogeny of birds are still resolved controversially in different analyses or are considered unresolvable. To address this problem, we produced an avian tree of life, which includes molecular
Circulating ribonucleic acids and metabolic stress parameters may reflect progression of autoimmune or inflammatory conditions in juvenile type 1 diabetes.
Kocic G
TheScientificWorldJournal, 11, 1496-1508 (2011)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
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Questions

  1. When using Tri Reagent BD, do we need to add EDTA to blood samples to prevent coagulation?

    1 answer

    1. The Product Information available under More Documents or at this link (https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/436/845/t3809bul.pdf) provides the following instructions:

      I. Sample Preparation:

      1A. Serum: Add 0.25 ml of serum to 0.75 ml of TRI Reagent BD. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      1B. Whole Blood or Plasma: Add 0.2 ml of whole blood or plasma to 0.75 ml of TRI Reagent BD supplemented with 20 ml of 5 N acetic acid per 0.2 ml of whole blood or plasma. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      It is possible to use whole blood (blood that has been allowed to clot), serum, or plasma. Blood that has clotted would be referred to as a clot plus serum or simply as clotted blood. If you are freshly drawing the blood and adding immediately to the Tri Reagent, an anticoagulant would not be required. For serum samples, the blood is simply allowed to clot. The clot is spun down and serum is collected above the clot. If plasma is used, this indicates the blood is first drawn into either a tube containing an anticoagulant or drawn with a syringe and then transferred to a tube containing an anticoagulant. The blood is then centrifuged and the plasma is removed before adding to the Tri Reagent. We would not recommend allowing the blood to clot and then trying to add the clotted blood to the Tri Reagent.

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