SRP2083
WT-1 (-KTS) human
recombinant, expressed in insect cells, ≥60% (SDS-PAGE)
Szinonimák:
AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352200
NACRES:
NA.77
Az árazás és az elérhetőség jelenleg nem elérhető
Javasolt termékek
biológiai forrás
human
rekombináns
expressed in insect cells
Teszt
≥60% (SDS-PAGE)
Forma
frozen liquid
molekulatömeg
~55.6 kDa
kiszerelés
pkg of 5 μg
tárolási körülmény
avoid repeated freeze/thaw cycles
koncentráció
250 μg/mL
szín
clear colorless
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
tárolási hőmérséklet
−70°C
Géninformáció
human ... WT1(7490)
Általános leírás
Wilms tumor 1 (WT1) is encoded by the gene mapped to human chromosome 11p13.[1] The encoded protein is expressed at high levels in nephrons, but at low levels in gonads, visceral adipose tissue, mesothelium and bone marrow cells.[2][3] In addition, it is also abundantly expressed in renal podocytes in adults and in parietal epithelial cells during early days.[3]
Biokémiai/fiziológiai hatások
WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA compared to the +KTS form.
Wilms tumor 1 (WT1) is involved in various vertebrate developmental processes such as cell differentiation and proliferation, apoptosis and regulation of epithelial/mesenchymal characteristics.[2] It serves as a tumor suppressor gene but might also possess oncogenic property.[4] The encoded protein regulates mRNA splicing and interactions between protein. It is associated with cancers and as well as kidney function.[2] Mutations in the gene leads to Denys-Drash syndrome, resulting in renal and genitourinary tract abnormalities.[3] Deletion in the germline WT1 gene causes Wilms′ tumor. This gene is highly expressed in a various types of cancers.[4]
Fizikai forma
Clear and colorless frozen liquid solution
Elkészítési megjegyzés
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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D A Haber et al.
Science (New York, N.Y.), 262(5142), 2057-2059 (1993-12-24)
A human Wilms tumor cell line (RM1) was developed to test the tumor suppressor activity of WT1, a zinc finger transcription factor that is expressed in the developing human kidney and is mutationally inactivated in a subset of Wilms tumors.
The Role of WT1 in Embryonic Development and Normal Organ Homeostasis.
Wilm B and Mu?oz-Chapuli R
Methods in Molecular Biology, 1467, 23-39 (2016)
The CUG-translated WT1, not AUG-WT1, is an oncogene.
Lee K Y, et al.
Carcinogenesis, 38(12), 1228-1240 (2017)
D A Haber et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(21), 9618-9622 (1991-11-01)
The chromosome 11p13 Wilms tumor susceptibility gene WT1 appears to play a crucial role in regulating the proliferation and differentiation of nephroblasts and gonadal tissue. The WT1 gene consists of 10 exons, encoding a complex pattern of mRNA species: four
J A Kreidberg et al.
Cell, 74(4), 679-691 (1993-08-27)
In humans, germline mutations of the WT-1 tumor suppressor gene are associated with both Wilms' tumors and urogenital malformations. To develop a model system for the molecular analysis of urogenital development, we introduced a mutation into the murine WT-1 tumor
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