SRE0047

Proteinase K from Tritirachium album

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥30 units/mg protein, for molecular biology

• Szinonimák: Endopeptidase K
• CAS-szám: 39450-01-6
• Enzyme Commission szám: 3.4.21.64 (BRENDA, IUBMB)
• EC-szám: 254-457-8
• MDL-szám: MFCD00132129
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: fungus (Tritirachium album)
• Minőségi szint: 400
• grade: for molecular biology
• form: lyophilized powder
• specifikus aktivitás: ≥30 units/mg protein
• molekulatömeg: 28.93 kDa
• szennyeződések: ≤1 ppm DNA
• alkalmazás(ok): diagnostic assay manufacturing
• idegen aktivitás: DNAse, Nickase and RNAse, none detected
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Proteinase K is an extracellular endopeptidase. It is synthesized by the mold Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein with a molecular weight of 28,930 Da. Active proteinase K is characterized with an unhydrolysed protein chain and autolysed polypeptide chains.

Alkalmazás

Proteinase K catalyzes the hydrolysis of keratin.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biokémiai/fiziológiai hatások

Proteinase K catalyzes the hydrolysis of keratin.

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Egység definíció

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Biztonsági adatok

• Piktogramok: GHS08,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H319,H334,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable