P9249

Monoclonal Anti-p53−Biotin antibody produced in mouse

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

• MDL-szám: MFCD01864735
• UNSPSC kód: 12352203
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: biotin conjugate
• antitest forma: ascites fluid
• antitest terméktípus: primary antibodies
• klón: DO-1, monoclonal
• form: buffered aqueous solution
• molekulatömeg: antigen ~53 kDa
• faj reaktivitás: human
• koncentráció: ~1 mg/mL
• technika/technikák: flow cytometry: suitable; immunocytochemistry: 5-10 μg/mL using human A431; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using human A431 cell extracts
• izotípus: IgG2a
• UniProt elérési szám: P04637
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... TP53(7157)

Leírás

Általános leírás

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

Alkalmazás

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biokémiai/fiziológiai hatások

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Tárolás és stabilitás

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Egyéb megjegyzések

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)