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P6874

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-1, purified from hybridoma cell culture

Szinonimák:

Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352203
NACRES:
NA.41
konjugátum:
unconjugated
application:
ARR
ELISA (i)
FACS
ICC
IHC
IP
WB
klón:
DO-1, monoclonal
faj reaktivitás:
human
citations:
27
technika/technikák:
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells

biológiai forrás

mouse

Minőségi szint

200

konjugátum

unconjugated

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

DO-1, monoclonal

Forma

buffered aqueous solution

molekulatömeg

antigen ~53 kDa

faj reaktivitás

human

koncentráció

~2 mg/mL

technika/technikák

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells

izotípus

IgG2a

UniProt elérési szám

P04637

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... TP53(7157)

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Általános leírás

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.

Egyediség

Mouse anti-(p53) antibody reacts specifically with (p53) if human.

Immunogén

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

Alkalmazás

Anti-p53 antibody, Mouse monoclonal has been used in:
  • semi-dry western blotting
  • immunoblotting
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation
  • flow cytometry
  • chromatin immunoprecipitation (ChIP) assay

Biokémiai/fiziológiai hatások

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000166008
098M4833V
117M4866V
114M4751V

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COA keresése

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Mikael S Lindström et al.
PloS one, 5(3), e9578-e9578 (2010-03-12)
Disruption of the nucleolus often leads to activation of the p53 tumor suppressor pathway through inhibition of MDM2 that is mediated by a limited set of ribosomal proteins including RPL11 and RPL5. The effects of ribosomal protein loss in cultured
Hui Miao et al.
PLoS genetics, 15(5), e1008144-e1008144 (2019-05-16)
Long noncoding RNAs (lncRNAs) participate in various biological processes such as apoptosis. The function of lncRNAs is closely correlated with their localization within the cell. While regulatory potential of many lncRNAs has been revealed at specific subcellular location, the biological
Samantha J McDonnell et al.
Cell death discovery, 5, 132-132 (2019-09-12)
Specific molecular interactions that underpin the switch between ER stress-triggered autophagy-mediated cellular repair and cellular death by apoptosis are not characterized. This study reports the unexpected interaction elicited by ER stress between the plasma membrane (PM)-localized apoptosis effector PERP and
Knockdown of hnRNPK leads to increased DNA damage after irradiation and reduces survival of tumor cells
Wiesmann N, et al.
Carcinogenesis, 38(3), 321-328 (2017)
D V Bulavin et al.
The EMBO journal, 18(23), 6845-6854 (1999-12-03)
Components of the ras signaling pathway contribute to activation of cellular p53. In MCF-7 cells, p38 kinase activated p53 more effectively than other members of the ras pathway. p53 and p38 kinase exist in the same physical complex, and co-expression
View All Related Papers

Cikkek

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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