Ugrás a tartalomra
Merck

H0412

Sigma-Aldrich

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Szinonimák:

PH3 Antibody - Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit, Ph3 Antibody, Anti-H3S10p

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

rabbit

Minőségi szint

konjugátum

unconjugated

antitest forma

IgG fraction of antiserum

antitest terméktípus

primary antibodies

klón

polyclonal

form

buffered aqueous solution

molekulatömeg

antigen 17 kDa

faj reaktivitás

Caenorhabditis elegans, bovine, human, rat, chicken, Drosophila, frog, mouse

technika/technikák

indirect immunofluorescence: 1:500 using human epitheloid carcinoma HeLa cell line treated with nocodazole.
microarray: suitable
western blot: 1:2,000 using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole.

UniProt elérési szám

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

phosphorylation (pSer10)

Általános leírás

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the PSR-45 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with phosphoserine conjugated to KLH. Histone proteins H3, H4, H2A, H2B and H1 function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibres. The gene encoding Histone H3 is mapped to human chromosome 1q42.12.
Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody recognizes histone H3 phosphorylated on Ser10 (17 kDa). Staining of pSer10-histone H3 in immunoblotting is specifically inhibited with the [pSer10] histone H3 immunizing peptide (human, amino acids 7-20). No inhibition is observed with the respective non-phosphorylated histone H3 peptide histone H3 (human, amino acids 7-20).

Immunogen

synthetic, phosphorylated peptide (pSer10) histone H3 (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species.

Alkalmazás

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit has been used in the detection of pSer10-histone H3 by immunoblotting, immunohistochemistry and by immunofluorescence.
Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody is used to tag Ser10 phosphorylated histone H3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting and immunofluorescence. It is used as a probe to determine the presence and roles of Ser10 phosphorylated histone H3 in chromosome condensation during both mitosis and meiosis.

Biokémiai/fiziológiai hatások

Phosphorylation of histone H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3s and occurs primarily in response to mitogenic and stress stimuli. Histone H3 is phosphorylated during mitosis on at least two serine residues, Ser10 and Ser28. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Members of the Aurora/AIK family of protein kinases are involved in the mitotic phosphorylation of histone H3 at Ser10 residue. Expression of Aurora-A and Aurora-B is tightly coordinated with histone H3 phosphorylation at G2/M transition and both kinases efficiently phosphorylate H3 at Ser10 in vitro and in vivo. Phosphorylation of H3 at Ser10 is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. Protein kinase A (PKA), ribosomal protein S6 kinase-3 (Rsk-2) and mitogen and stress activated protein kinase-1 (Msk-1) are necessary for the histone H3 phosphorylation.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Nem találja a megfelelő terméket?  

Próbálja ki a Termékválasztó eszköz. eszközt

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Phosphorylation of histone H3 correlates with transcriptionally active loci
Nowak SJ and Corces VG
Genes & Development, 14(23), 3003-3013 (2000)
Phosphoacetylation of histone H3 on c-fos-and c-jun-associated nucleosomes upon gene activation
Clayton AL, et al.
The Embo Journal, 19(14), 3714-3726 (2000)
AMOTL1 promotes breast cancer progression and is antagonized by merlin
Couderc C, et al.
Neoplasia, 18(1), 10-24 (2016)
Mitotic phosphorylation of histone H3: spatio-temporal regulation by mammalian Aurora kinases
Crosio C, et al.
Molecular and Cellular Biology, 22(3), 874-885 (2002)
Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy
Schaefer IM, et al.
Cancer Cytopathology, 126(8), 552-566 (2018)

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással