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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

Szinonimák:

Protease inhibitor solution

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00677817
UNSPSC kód:
12352200
NACRES:
NA.77
Forma:
DMSO solution
tárolási hőmérséklet:
−20°C

Terméknév

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

biológiai forrás

synthetic

Minőségi szint

300

Forma

DMSO solution

tárolási hőmérséklet

−20°C

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Általános leírás

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Egyediség

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Alkalmazás

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Tulajdonságok és előnyök

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Komponensek

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Mennyiség

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Elkészítési megjegyzés

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

kapcsolódó termék

Product No.
Leírás
Árazás

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8820

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

S8830

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

185.0 °F - closed cup

Lobbanási pont (C)

85 °C - closed cup

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000305631
0000226095
0000226094
039M4073V

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COA keresése

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Dokumentumtár megtekintése

E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of
Shuji Kaneko et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(1), 82-92 (2002-01-05)
The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
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Neuroscience, 256, 456-466 (2013-10-26)
Nogo-A is a transmembrane protein originally discovered in myelin, produced by postnatal CNS oligodendrocytes. Nogo-A induces growth cone collapse and inhibition of axonal growth in the injured adult CNS. In the intact CNS, Nogo-A functions as a negative regulator of
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The EMBO journal, 20(7), 1765-1773 (2001-04-04)
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear loci and one chloroplast gene, tscA, are needed for this process.
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