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S8830

Sigma-Aldrich

Protease Inhibitor Cocktail

SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins

Szinonimák:

Protease Inhibitor Cocktail

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

MDL-szám:
MFCD00677817
UNSPSC kód:
12352200
NACRES:
NA.77

product name

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins

biológiai forrás

bovine lung

Minőségi szint

200

form

tablet

tárolási hőmérséklet

2-8°C

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Általános leírás

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection.

Egyediség

Inhibits serine, cysteine, aspartic, and metalloproteases.

Alkalmazás

The broad specificity of SIGMAFAST™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select media.

Tulajdonságok és előnyök

EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.

Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.

Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.

High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Komponensek

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin

Mennyiség

One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Fizikai forma

Tablet that is water soluble

Tárolás és stabilitás

The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Jogi információk

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Piktogramok

Corrosion
GHS05

Figyelmeztetés

Danger

Figyelmeztető mondatok

H314

Veszélyességi osztályok

Eye Dam. 1 - Skin Corr. 1A

Tárolási osztály kódja

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Benjamin M Nitsche et al.
BMC genomics, 13, 380-380 (2012-08-10)
Filamentous fungi are confronted with changes and limitations of their carbon source during growth in their natural habitats and during industrial applications. To survive life-threatening starvation conditions, carbon from endogenous resources becomes mobilized to fuel maintenance and self-propagation. Key to
Zilun Wei et al.
Life sciences, 232, 116632-116632 (2019-07-07)
The inflammation modulation effects of mesenchymal stromal cell-derived exosomes (MSC-EXO) are well established. We aimed to explore the mechanism behind the inflammatory responses of numerous exosomal cargo molecules that have been neglected in molecular biology research, and to develop an
Katarzyna Rybak et al.
Developmental cell, 29(5), 607-620 (2014-06-03)
Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood.
Stephan G Mauracher et al.
Phytochemistry, 99, 14-25 (2014-01-28)
Tyrosinases catalyze two initial reaction steps in the formation of melanin. Purification of tyrosinases had always been a process accompanied with various problems caused by enzymatic browning processes. Here, an approach is presented for the purification of the latent enzyme
Jeremy Celver et al.
Journal of neurochemistry, 120(1), 56-69 (2011-11-01)
Detergent-resistant membranes (DRM) are thought to contain structures such as lipid rafts that are involved in compartmentalizing cell membranes. We report that the majority of D(2)-dopamine receptors (D(2)R) expressed endogenously in mouse striatum or expressed in immortalized cell-lines is found
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Cikkek

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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