Összes fotó(1)
Fontos dokumentumok
P5704
Monoclonal Anti-Protein Kinase C (PKC) antibody produced in mouse
clone MC5, ascites fluid
Szinonimák:
Monoclonal Anti-Protein Kinase C, Anti-PKC
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
konjugátum:
unconjugated
application:
ARR
IHC (f)
IP
WB
IHC (f)
IP
WB
klón:
MC5, monoclonal
faj reaktivitás:
rat, bovine, mouse, human
citations:
66
technika/technikák:
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500 using rat brain cytosol preparation
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500 using rat brain cytosol preparation
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
unconjugated
antitest forma
ascites fluid
antitest terméktípus
primary antibodies
klón
MC5, monoclonal
molekulatömeg
antigen 80 kDa
tartalmaz
15 mM sodium azide
faj reaktivitás
rat, bovine, mouse, human
technika/technikák
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500 using rat brain cytosol preparation
izotípus
IgG2a
UniProt elérési szám
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... PRKCB(5579) , PRKCZ(5590)
mouse ... Prkcb(18751) , Prkcc(18752)
rat ... Prkcb(25023) , Prkcg(24681)
Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz
Általános leírás
Protein Kinase C (PKC) is a phospholipid and calcium dependent enzyme that regulates intracellular signal transduction. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity, and release of neurotransmitters. PKC inhibitors have been implicated in diabetic retinopathy . Monoclonal Anti-Protein Kinase C (clone MC5) recognizes an epitope located within the amino acid sequence 296-317 at the hinge region (close to or at the trypsin cleavage site) of PKC . The antibody is specific for PKC in humans, bovines, rats and mice. Binding of the antibody to in vitro purified PKC blocks trypsin-mediated partial proteolysis. Phorbol-ester induced down-regulation of the kinase is inhibited by the introduction of Fab fragment of the antibody into a rodent glioma cell line .
Protein kinase C (PKC) is a 77-90kD protein that belongs to serine/threonine protein kinases family. It has a crucial role in cellular regulation, signal transduction and tumor promotion and oncogenesis. Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for identification and localization of PKC in normal and malignant tissues. It can also be used in immunoblotting (diluted 1:500) using rat brain extracts. Mouse Anti-Protein Kinase C (PKC) antibody reacts specifically with PKC polypeptide (80kD). The product has also shown cross reactivity for rat, bovine, mouse and human.
Egyediség
Reacts with the 80 kDa polypeptide of PKC. Binding of the antibody to purified kinase in vitro, blocks partial proteolysis by trypsin. Introduction of Fab fragments of the antibody into a rodent glioma cell line inhibits phorbol-ester-induced down-regulation of the kinase.
Immunogén
purified bovine brain protein kinase C
Alkalmazás
Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for the localization of protein kinase C by using different immunochemical assays like- dot blot, immunoprecipitation and ELISA. It can also be used as a primary antibody in immunohistochemistry (diluted 1:200) .
Monoclonal Anti-Protein Kinase C (PKC) antibody is suitable for use in immunohistochemistry (frozen sections), immunoblot (80 kD, using bovine brain PKC, extracts of rat glioma and murine NIH 3T3 cell lines), dot blot, ELISA, microarray and immunoprecipitation (rat glioma and human cell extracts/lysates).
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
Nem találja a megfelelő verziót?
Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Zi-Li Yu et al.
International journal of molecular medicine, 37(2), 468-474 (2016-01-09)
Lysophosphatidic acid (LPA) is an efficient, bioactive phospholipid involved in various biological processes. In this study, LPA-induced connective tissue growth factor (CTGF/CCN2) expression and the underlying mechanisms were investigated using the MC3T3-E1 cell line. The MC3T3-E1 cells were stimulated with
Influence of intrathecal injection with dexmedetomidine on the
behavioral ability and analgesic effects on rats with neuropathic
pain and expression of protein kinase C in the spinal dorsal horn
behavioral ability and analgesic effects on rats with neuropathic
pain and expression of protein kinase C in the spinal dorsal horn
Li X and Zhang W
Experimental and Therapeutic Medicine, 16(5), 3835-3840 (2018)
M I López Gálvez
Current pharmaceutical biotechnology, 12(3), 386-391 (2010-10-14)
To review current data regarding protein Kinase C beta (PKCß) inhibitors and its efficacy in reducing the burden of diabetic retinopathy (DR) and diabetic macular edema (DME). MEDLINE search (1980-2009) and presentations to major meetings. DR and DME have emerged
Lorenzo Cangiano et al.
PloS one, 2(12), e1327-e1327 (2007-12-20)
Hyperpolarization-activated cyclic nucleotide-sensitive (HCN) channels mediate the I(f) current in heart and I(h) throughout the nervous system. In spiking neurons I(h) participates primarily in different forms of rhythmic activity. Little is known, however, about its role in neurons operating with
Akira Hara et al.
Stem cells and development, 17(4), 619-627 (2008-04-09)
In vivo transplantation of undifferentiated embryonic stem (ES) cells can produce teratomas with uncontrolled cell proliferation. Although ES cells may be attractive candidates for human cell-replacement therapy in the future, the major limitation of its application to the therapy is
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással