Összes fotó(1)
Fontos dokumentumok
P3803
Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA
for molecular biology
Szinonimák:
DNA extraction
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12190000
NACRES:
NA.52
grade:
for molecular biology
Teszt:
≥99.0%
alkalmazás(ok):
agriculture
Javasolt termékek
grade
for molecular biology
Minőségi szint
Teszt
≥99.0%
Forma
liquid
pH
6.5-6.9(pH of phenolic phase)
alkalmazás(ok)
agriculture
tárolási hőmérséklet
2-8°C
Alkalmazás
Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.
Figyelmeztetés
If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.
Egyéb megjegyzések
This product has a shelf life of two years.
Javasolt termékek
For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
Célzott szervek
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Tárolási osztály kódja
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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