77617

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

• CAS-szám: 136112-00-0
• MDL-szám: MFCD00133763
• UNSPSC kód: 12352401
• PubChem Substance ID: 329767815
• NACRES: NA.25
• grade: for molecular biology
• technika/technikák: ChIP: suitable; DNA extraction: suitable; DNA purification: suitable
• alkalmazás(ok): agriculture

Tulajdonságok

• grade: for molecular biology
• Minőségi szint: 200
• termékcsalád: BioUltra
• Forma: liquid
• technika/technikák: ChIP: suitable; DNA extraction: suitable; DNA purification: suitable
• szennyeződések: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 7.2-8.3(H₂O-phase, after extraction with H₂O, 1:1)
• sűrűség: 1.28 g/mL at 20 °C
• Abszorpció: cut-off at 360 nm
• alkalmasság: suitable for molecular biology
• alkalmazás(ok): agriculture
• tárolási hőmérséklet: 2-8°C
• SMILES string: ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
• InChI: 1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
• Nemzetközi kémiai azonosító kulcs: ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Leírás

Általános leírás

Phenol-chloroform-isoamyl alcohol mixture (PCI-mixture) is a widely utilized mixture in molecular biology and biochemical research, specifically designed for nucleic acid purification, including DNA and RNA. With its essential composition of 25% phenol, 24% chloroform, and 1% isoamyl alcohol, PCI-mixture plays a pivotal role in separating proteins from nucleic acids during the purification process. Phenol denatures proteins, chloroform creates a distinct layer to trap them, and isoamyl alcohol facilitates the clear separation of these layers, enabling the isolation of purified DNA or RNA in the aqueous phase.
The applications of PCI-mixture are diverse, encompassing RNA isolation, where it efficiently extracts RNA from animal and plant tissues for downstream analyses like reverse transcription and gene expression studies. Further, PCI-mixture also proves instrumental in DNA extraction, enabling efficient recovery for various applications such as DNA minisatellite fingerprinting for genetic identification. As a general purification technique, PCI-mixture ensures high-quality preparations of both DNA and RNA for further research.

Alkalmazás

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.Further, also in the extraction of DNA for the generation of DNA minisatellite fingerprints

Biokémiai/fiziológiai hatások

When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.

Tulajdonságok és előnyök

• Versatile and adaptable for a wide variety of laboratory and research applications
• Free from DNase, RNase, NICKase and protease

Fizikai forma

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Egyéb megjegyzések

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Biztonsági adatok

• Piktogramok: GHS06,GHS08,GHS05,GHS09
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H301 + H331,H312,H314,H336,H341,H351,H361d,H372,H373,H411
• Óvintézkedésre vonatkozó mondatok: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Veszélyességi osztályok: Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
• Célzott szervek: Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
• Tárolási osztály kódja: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Lobbanási pont (F): 174.2 °F
• Lobbanási pont (C): 79 °C
• Egyéni védőeszköz: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter