OGS3213
PSF-CMV-PURO-NH2-FLAG® - N-TERMINAL FLAG® TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Szinonimák:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352200
NACRES:
NA.85
Javasolt termékek
tag
FLAG® tagged
form
buffered aqueous solution
molekulatömeg
size 6173 bp
baktériumok kiválasztása
kanamycin
emlős sejtek kiválasztása
puromycin
Replikáció eredete
pUC (500 copies)
Peptidhasítás
no cleavage
Peptid tag helye
N-terminal
promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
riporter gén
none
kiszállítva
ambient
tárolási hőmérséklet
−20°C
Általános leírás
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Szekvencia
To view sequence information for this product, please visit the product page
Analízis megjegyzés
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Jogi információk
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
12 - Non Combustible Liquids
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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