T6824

pPolh-FLAG-1 Transfer Vector

• Szinonimák: ddddk tag, dykddddk tag
• UNSPSC kód: 41106621
• NACRES: NA.32

Tulajdonságok

• Minőségi szint: 200
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.

Alkalmazás

pPolh-FLAG-1 is a 5575 bp baculovirus transfer vector used for producing FLAGÒ fusion proteins in insect cells. The vector is designed to be compatible with most baculoviral DNA systems that require the essential gene ORF1629 for complementation of lethal deletions and the recovery of viable recombinant virus. It is also a transfer vector for generation of recombinant Baculovirus and expression of N-terminal FLAG^® fusions in insect cells.

Jogi információk

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)