Összes fotó(1)
Fontos dokumentumok
MTOXCE3A4
CypExpress™ 3A4 Cytochrome P450 human
Szinonimák:
CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450 3A4, P450C3, P450PCN1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
biológiai forrás
human
rekombináns
expressed in Pichia pastoris
form
dry powder
UniProt elérési szám
alkalmazás(ok)
cell analysis
kiszállítva
dry ice
tárolási hőmérséklet
−70°C
Géninformáció
human ... CYP3A4(1576)
Alkalmazás
Poster: A Novel, Robust Recombinant Human P450 Biocatalytic System
Poster: Evaluation of Dry Powder Preparations of Permeabilized
Pichia pastoris Cells Expressing Human Cytochrome P450 2D6 Enzyme (CYP2D6) for Drug Metabolite Generation
Poster: Facile Scale-up Biocatalytic Production of P-450 Metabolites Using a Novel Recombinant Human Catalytic System
Poster: Multi-Cycle Production of Metabolites with human P450 systems
Poster: Rapid identifcation and production of metabolites using CypExpress™ 2D6m 3A4 and 2C9
Poster: Rapid identifcation and production of metabolites using stabilized dried powder (SDP) of human cytochrome P450S (hCYPS) 2D6, 3A4, and 2C9 engineered in yeast
Poster: Utilizing a Novel Biocatalytic Human P450 System for the Multi - Cycle Production of Metabolites
Poster: Evaluation of Dry Powder Preparations of Permeabilized
Pichia pastoris Cells Expressing Human Cytochrome P450 2D6 Enzyme (CYP2D6) for Drug Metabolite Generation
Poster: Facile Scale-up Biocatalytic Production of P-450 Metabolites Using a Novel Recombinant Human Catalytic System
Poster: Multi-Cycle Production of Metabolites with human P450 systems
Poster: Rapid identifcation and production of metabolites using CypExpress™ 2D6m 3A4 and 2C9
Poster: Rapid identifcation and production of metabolites using stabilized dried powder (SDP) of human cytochrome P450S (hCYPS) 2D6, 3A4, and 2C9 engineered in yeast
Poster: Utilizing a Novel Biocatalytic Human P450 System for the Multi - Cycle Production of Metabolites
Biokémiai/fiziológiai hatások
Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Ketoconazole, itraconazole, azamulin, troleandomycin and verapamil are inhibitors, whereas rifampin is an inducer of the enzyme. The isozyme CYP3A4 is a major pathway for the invivo conversion of testosterone to 6β-Hydroxytestosterone via hydroxylation.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds like xenobiotics, pollutants and drug molecules. Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes.
Tulajdonságok és előnyök
CypExpress™ 3A4 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 3A4 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress™ are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress™ retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.
Egység definíció
A reaction mixture containing 500 μM testosterone and 100 mg/mL of CypExpress™ 3A4 will produce 121.9 μM 6b-hydroxytestosterone in 3 hours at 30°C under the conditions described.
Fizikai forma
Light yellow, very fine powder
Feloldás
Per Protocol/Variable
Jogi információk
6538 is a trademark of American Type Culture Collection
CypExpress is a trademark of Oxford Biomedical Research
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az ügyfelek ezeket is megtekintették
Pharmacokinetic-pharmacodynamic consequences and clinical relevance of cytochrome P450 3A4 inhibition
Dresser, et al.
Clinical Pharmacokinetics, 38(1), 41-57 (2000)
Crystal structures of human cytochrome P450 3A4 bound to metyrapone and progesterone
Williams PA, et al.
Science, 305(5684), 683-686 (2004)
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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