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Merck

H7411

Sigma-Aldrich

Anti-HA−FITC antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

Szinonimák:

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.56

biológiai forrás

mouse

Minőségi szint

konjugátum

FITC conjugate

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

HA-7, monoclonal

form

buffered aqueous solution

tárolási körülmény

protect from light

koncentráció

1 mg/mL

technika/technikák

immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

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Általános leírás

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Egyediség

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Alkalmazás

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
  • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
  • in western blotting detection of spindle-associated proteins in Trypanosoma brucei
  • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biokémiai/fiziológiai hatások

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

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Zhou Q, et al.
Nucleic Acids Research, 46(16), 8216-8231 (2018)
Use of FITC-labeled influenza virus and flow cytometry to assess binding and internalization of virus by monocytes-macrophages and lymphocytes
Nichols JE, et al.
Archives of Virology, 130(3-4), 441-455 (1993)
Annalena La Porte et al.
Journal of virology, 90(21), 9889-9904 (2016-08-26)
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Aortic aneurysm generation in mice with targeted deletion of integrin-linked kinase in vascular smooth muscle cells
Shen D, et al.
Circulation Research, 109(6), 616-628 (2011)
Irina Kufareva et al.
Methods in enzymology, 570, 389-420 (2016-02-29)
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