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H5791

Sigma-Aldrich

Hepatocyte Growth Factor human

HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Szinonimák:

HGF, Hepatopoietin A, Scatter Factor

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00217422
UNSPSC kód:
12352202
NACRES:
NA.77

product name

Hepatocyte Growth Factor human, HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

biológiai forrás

human

Minőségi szint

100

rekombináns

expressed in HEK 293 cells

Teszt

≥95% (SDS-PAGE)

form

lyophilized powder

hatékonyság

≤20 ng/mL EC50

minőség

endotoxin tested

molekulatömeg

monomer 70 kDa (glycosylated)

kiszerelés

pkg of 5x10 μg
pkg of 10 μg

tárolási körülmény

avoid repeated freeze/thaw cycles

technika/technikák

cell culture | mammalian: suitable

szennyeződések

≤1 EU/mg

UniProt elérési szám

P14210

tárolási hőmérséklet

−20°C

Géninformáció

human ... HGF(3082)

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Általános leírás

The HGF gene spans ∼70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of human chromosome 7, 7q21.1.

Biokémiai/fiziológiai hatások

HGF is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. It is a potent mitogen, stimulating the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells), and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells, and HepG2 (human hepatoma) cells. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Egyéb megjegyzések

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Fizikai forma

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Elkészítési megjegyzés

HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analízis megjegyzés

The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.

Jogi információk

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

T Nakamura et al.
Nature, 342(6248), 440-443 (1989-11-23)
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification
Karène Mahtouk et al.
Biochimica et biophysica acta, 1806(2), 208-219 (2010-07-27)
Hepatocyte growth factor (HGF) and its receptor MET are essential during embryonic development and throughout postnatal life. However, aberrant MET activation, due to overexpression, mutations, or autocrine ligand production, contributes to the development and progression of a variety of human
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
Hiroshi Kusunoki et al.
Hypertension research : official journal of the Japanese Society of Hypertension, 37(6), 500-506 (2014-03-14)
The paracrine loop involving inflammatory cytokines between adipocytes and macrophages establishes a vicious cycle that aggravates pro-inflammatory changes in adipose tissue. The serum level of hepatocyte growth factor (HGF) is increased in metabolic syndrome, but whether HGF is beneficial or
Yohei Ozawa et al.
BMC cancer, 15, 451-451 (2015-06-04)
c-Met is widely known as a poor prognostic factor in various human malignancies. Previous studies have suggested the involvement of c-Met and/or its ligand, hepatocyte growth factor (HGF), in esophageal squamous cell carcinoma (ESCC), but the correlation between c-Met status
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