05-598
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002
clone RR002, Upstate®, from mouse
Szinonimák:
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
RR002, monoclonal
application:
ICC
IHC
WB
IHC
WB
faj reaktivitás:
vertebrates, nematode, Caenorhabditis elegans, human
technika/technikák:
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
42
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
RR002, monoclonal
faj reaktivitás
vertebrates, nematode, Caenorhabditis elegans, human
gyártó/kereskedő neve
Upstate®
technika/technikák
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotípus
IgG
alkalmasság
not suitable for ELISA applications
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
phosphorylation (pSer10)
Géninformáció
human ... HIST1H3F(8968)
Általános leírás
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Egyediség
Broad species cross-reactivity expected due to sequence conservation.
This antibody recognizes Histone H3 phosphorylated at Serine 10.
Immunogén
Peptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.
Alkalmazás
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.
ELISA Analysis:
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.
Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.
Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.
Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.
Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
Minőség
Routinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.
Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
Cél megnevezése
17 kDa
Fizikai forma
Format: Purified
Purified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Analízis megjegyzés
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Joao P Lopes et al.
Aging cell, 9(1), 64-77 (2009-11-10)
Cdk5 dysregulation is a major event in the neurodegenerative process of Alzheimer's disease (AD). In vitro studies using differentiated neurons exposed to Abeta exhibit Cdk5-mediated tau hyperphosphorylation, cell cycle re-entry and neuronal loss. In this study we aimed to determine
Protein phosphatase 2A activity affects histone H3 phosphorylation and transcription in Drosophila melanogaster
Nowak, S. J., et al
Molecular and cellular biology, 23, 6129-6138 (2003)
Transmembrane potential of GlyCl-expressing instructor cells induces a neoplastic-like conversion of melanocytes via a serotonergic pathway.
Blackiston, D; Adams, DS; Lemire, JM; Lobikin, M; Levin, M
Disease models & mechanisms null
Functional consequences of cyclin D1/BRCA1 interaction in breast cancer cells.
Kehn, K; Berro, R; Alhaj, A; Bottazzi, ME; Yeh, WI; Klase, Z; Van Duyne, R; Fu, S; Kashanchi, F
Oncogene null
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment
Saccani, S., et al
Nature Immunology, 3, 69-75 (2002)
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