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Key Documents

GE17-5442-02

Ficoll®-Paque Premium

Cytiva 17-5442-02, pack of 6 × 100 mL

Szinonimák:

Ficoll density gradient medium, cell culture media

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352201
NACRES:
NA.25

form

liquid

kiszerelés

pack of 6 × 100 mL

gyártó/kereskedő neve

Cytiva 17-5442-02

szín

Colorless to slightly yellow

pH-tartomány

5.5-7.5

Related Categories

Általános leírás

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

Alkalmazás

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Tulajdonságok és előnyök

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Tárolás és stabilitás

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analízis megjegyzés

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Jogi információk

Ficoll is a registered trademark of Cytiva

Tárolási osztály kódja

12 - Non Combustible Liquids

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Xin Ni et al.
PloS one, 12(9), e0184773-e0184773 (2017-09-15)
Inflammation has been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Connexins (Cxs)-based gap junction channels (GJCs) or hemichannels (HCs) are involved in the maintenance of homeostasis in the immune system. However, the
Avanish K Varshney et al.
PloS one, 13(1), e0190537-e0190537 (2018-01-25)
Staphylococcus aureus can cause devastating and life-threatening infections. With the increase in multidrug resistant strains, novel therapies are needed. Limited success with active and passive immunization strategies have been attributed to S. aureus immune evasion. Here, we report on a

Cikkek

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads facilitate Percoll gradient monitoring in biological separations.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocols

Recommended Standard Method for Isolating Mononuclear Cells

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

Ajánlott standard módszer a mononukleáris sejtek izolálására

Az alábbi eljárást a Ficoll-Paque PLUS-szal értékelték, és a maximális reprodukálhatóság érdekében normál vérminták elválasztására ajánlott.

Hogyan készítsünk és használjunk Percoll gradienseket?

A Percoll-gradiens elkészítéséhez a Percoll ozmolalitását sóoldattal vagy sejttenyésztő közeggel kell beállítani, hogy a Percoll izotóniás legyen a fiziológiás sóoldatokkal.

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Related Content

Applications of Percoll In Blood Cells

Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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