E1388

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Szinonimák: ECGS, β-ECGF
• CAS-szám: 105843-51-4
• MDL-szám: MFCD00131012
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• product name: β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
• biológiai forrás: human
• Minőségi szint: 200
• rekombináns: expressed in E. coli
• Teszt: ≥97% (SDS-PAGE)
• form: lyophilized powder
• hatékonyság: 0.03-0.3 ng/mL ED₅₀/EC₅₀
• minőség: endotoxin tested
• molekulatömeg: predicted mol wt ~17 kDa
• kiszerelés: pkg of 25 μg
• tárolási körülmény: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: ≤1.0 EU/μg protein Endotoxin level (LAL test)
• UniProt elérési szám: P49765
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... VEGFB(7423)

Leírás

Általános leírás

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Alkalmazás

β-Endothelial Cell Growth Factor human has been used:

• in cell death assay
• in endothelial cell culture
• in human umbilical vascular endothelial cell (HUVEC) culture

Biokémiai/fiziológiai hatások

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Egyéb megjegyzések

14 amino acid, N-terminal extended form of human FGF-acidic.

Fizikai forma

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analízis megjegyzés

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)