E2759

Endothelial cell growth supplement from bovine neural tissue

powder, suitable for cell culture

• Szinonimák: ECGS, Endothelial Mitogen
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• product name: Endothelial cell growth supplement from bovine neural tissue, ECGS, suitable for cell culture
• biológiai forrás: bovine neuroblastoma
• Minőségi szint: 200
• form: powder
• hatékonyság: 2-200 μg/mL ED₅₀/EC₅₀
• minőség: endotoxin tested
• kiszerelés: pkg of 15 and 5X15 mg
• tárolási körülmény: avoid repeated freeze/thaw cycles
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: ≤20 EU/vial (LAL test)
• szín: tan to brown
• oldhatóság: water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF).

Alkalmazás

Endothelial cell growth supplement from bovine neural tissue has been used as a supplement in Dulbecco′s modified Eagle′s medium supplement and F-12K media supplement for human umbilical vein endothelial cells culture.

Biokémiai/fiziológiai hatások

Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC). It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs). It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation. An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Elkészítési megjegyzés

ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Analízis megjegyzés

The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Biztonsági adatok

• Tárolási osztály kódja: 13 - Non Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)