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Merck

CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Szinonimák:

Mitochondrial activity assay

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352207
NACRES:
NA.75

kiszerelés

pkg of 1 kit

Minőségi szint

technika/technikák

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

alkalmazás(ok)

cell analysis
detection

észlelési módszer

colorimetric

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Általános leírás

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Alkalmazás

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Kiszerelés

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

kapcsolódó termék

Product No.
Leírás
Árazás

Figyelmeztetés

Danger

Figyelmeztető mondatok

Veszélyességi osztályok

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

Célzott szervek

Central nervous system

Tárolási osztály kódja

3 - Flammable liquids

Lobbanási pont (F)

53.6 °F - closed cup

Lobbanási pont (C)

12 °C - closed cup


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

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Carla M R Lacerda et al.
American journal of physiology. Heart and circulatory physiology, 302(10), H1983-H1990 (2012-02-22)
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Cikkek

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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