C7555

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type VI, saline suspension

• Szinonimák: ConA
• UNSPSC kód: 12161501
• NACRES: NA.32

Tulajdonságok

• biológiai forrás: Canavalia ensiformis
• Minőségi szint: 200
• konjugátum: agarose conjugate
• típus: Type VI
• Forma: saline suspension
• a címkézés mértéke: 15-30 mg concanavalin A protein per mL
• Mátrix: cross-linked 4% beaded agarose
• mátrix aktiválás: cyanogen bromide
• mátrix csatolmány: amino
• mátrix távtartó: 1 atom
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Concanavalin A (Con A), isolated from Jack beans (Canavalia ensiformis), is a mannose or glucose specific-binding lectin. The lectin is a tetramer and each subunit possesses a saccharide-binding site. Con A can be isolated by affinity chromatography with dextrans.

Alkalmazás

Concanavalin A (Con A) has been used for enzyme immobilization.

Used for the purification of glycoproteins containing mannose

Biokémiai/fiziológiai hatások

Concanavalin A (Con A) is a T cell mitogen that can activate the lymphocytes.
Con A can induce tumor cell death through autophagy.

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Fizikai forma

Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl₂, 1 mM MgCl₂ and 1 mM MnCl₂ containing preservative

Analízis megjegyzés

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H317,H334
• Óvintézkedésre vonatkozó mondatok: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Veszélyességi osztályok: Resp. Sens. 1 - Skin Sens. 1
• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable